A case presentation for positive SARS-CoV-2 RNA recurrence in a patient with a history of type 2 diabetes that had recovered from severe COVID-19
Coronavirus disease in 2019 (COVID-19) considered the spread primarily by people who have tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we discuss COVID 19 patients with severe and a history of type 2 diabetes who have a positive recurrence of SARS-CoV ribonucleic acid-2 (RNA) after recovering.
Patients initially discharged after two consecutive negative SARS-CoV-2 test RNA and partially absorbed bilateral lesions in chest computed tomography (CT). However, at the first follow-up, reverse transcription-polymerase chain reaction (RT-PCR) assay with oropharyngeal swab samples positive for SARS-CoV-2. However, he did not show obvious clinical symptoms and enhanced CT of the chest. Patients prescribed anti-viral drugs. Eight consecutive test RT-PCR on oropharyngeal swab specimens made after he re-admitted in our hospital.
Positive results in 12, 14, 19, 23 and 26 March and negative on March 28, and 6 and 12 of April. After the second discharge, he has tested negative for 5 weeks. This case highlights the importance of active surveillance of SARS-CoV-2 RNA during follow-up period so that an assessment can be made of infectivity. Keywords: Coronavirus disease in 2019; Relapse; SARS-CoV-2; Weight; Type 2 diabetes.
A case presentation for positive SARS-CoV-2 RNA recurrence in a patient with a history of type 2 diabetes that had recovered from severe COVID-19
Genetic diversity of Ehrlichia strains ruminantium of farm fields selected in South Africa
Heartwater is a tick-borne disease caused by Ehrlichia ruminantium intracellular rickettsial parasite and transmitted by ticks Amblyomma hebraeum. Heartwater problematic in endemic areas because it causes high mortality in ruminants and lead to economic losses that threaten productivity and food security. This may indicate that there is a plus genetic diversity in the field, which can result in a more virulent isolates than Ball3 and Welgevonden isolates. The genetic diversity of E. ruminantium investigated in this study, focusing on the region of the gene polymorphic pCS20 and four open reading frames (ORFs) were identified by subtractive hybridization.
16S ribosomal ribonucleic acid genes were confirmed E. ruminantium in the brain, blood and tick samples of genomic deoxyribonucleic acid (n = 3792) were collected from 122 farms were randomly selected from seven provinces of South Africa where heartwater endemic. The conserved E. ruminantium pCS20 quantitative polymerase chain reaction (qPCR) assay was used to scan all field samples were collected. A total of 433 samples tested positive by qPCR using local pCS20 genes, 167 were sequenced. Stocks are known and field samples were analyzed, and the resulting phylogenetic tree of the consensus sequence.
A total of 25 new clades identified; This, nine isolates from infected blood can be propagated in cell culture. These clades are not geographically limited to certain areas but distributed between heartwater-endemic regions in South Africa. Thus, knowledge of the diversity of strains of E. ruminantium very important for controlling heartwater and provide a basis for further vaccine development. Our results show that the size of the nucleus, chromatin organization and localization DAXX altered in breast cancer tissue compared with those in the control network. Compared with cytoplasmic and nuclear expression in benign breast tissue, DAXX it colocalized with promyelocytic leukemia in the core with granular distribution.
DAXX endogenous messenger ribonucleic acid levels are regulated in UV radiation in MDA-MB-231 cells. DAXX-deficient cells tend to be more sensitive to radiation than control cells. Instead, the cells exhibited reduced DAXX-overexpressing phosphorylated histone H2AX accumulation (-H2AX γ), increased cell survival, and resistance to UV-induced damage.
