New insights into the molecular mechanism of Boletus edulis ribonucleic acid fraction (BE3) concerning antiproliferative activity on human colon cancer cells.
One of the relatively new and promising cancer treatment strategies is chemoprevention, which involves the use of natural or synthetic compounds for blocking, inhibiting or reversing carcinogenesis. A valuable and unexploited source of chemo-recent compounds appears to be edible mushrooms belonging to higher baseidomycetes. Biolettus edulis biopolymers extracted with hot water and purified by anion exchange chromatography showed antiproliferative activity in the colon cancer cells, but only the BE3 fraction, mainly composed of ribonucleic acids, was capable of inhibiting the Synthesis of DNA in HT-29 cells.
The current work aims to elucidate the molecular mechanism of this fraction of ribonucleic acid Edulis Boletus and in this flow flow cytometry and Western blot has been applied to cell cycle analysis in HT-29 cells. We found that the antimmerificative capacity of the BE3 fraction observed in the HT-29 cells was associated with the expression modulation of cell cycle control proteins (cyclin D1, cyclin a, p21 and p27) resulting in cell accumulation in the phase S of the cell cycle. In addition, the BE3 fraction showed an effective silence of signal transduction in a MAPK / ERK channel in HT-29 and LS180 colon cancer cell lines.
Thus, the results previously and currently obtained indicate that the Be3 fraction of Boletus edulis has great potential and should be exploited in more detail through studies on animals and clinics to develop a new effective and safe therapeutic strategy for people threatened or suffered from colon cancer. The identification of extrapulmonary legionellosis is difficult because of the lack of clinical suspicion and limitations of conventional microbiological methods. We present a series of graft graft cases of hematopoietic cells with an extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid Sanger sequencing and laser desorption of the matrix / ionization of flight mass spectrometry.
Biomarker detection and categorization in the sequencing meta-analysis of ribonucleic acid using Bayesian hierarchical models.
The meta-analysis combining several transcriptomic studies increases the statistical power and the accuracy of the detection of genes expressed differentially. As new generation sequencing experiments become mature and affordable, a growing number of RNA-SEQ datasets are available in the public domain. Account data technology provides better experimental accuracy, reproducibility and unjust detection capacity. A naive approach to combining several RNA-SEQ studies consists of applying differential analysis tools such as EDGER and DESEQ to each study, and combine the summary statistics of p-values or effect sizes by methods. Classic meta-analysis. Such a two-step approach loses statistical power, especially for genes with long-term abundance or low expression.
In this article, we propose a full Bayesian hierarchical model (namely BayesMetaseq) for the meta-analysis of the RNA-SEQ by modeling the counting data, integrating information on genes and between studies and modeling of differential signals Potentially heterogeneous on studies by latent variables. A preliminary dirichlet process mixture is then applied to the latent variables to create a categorization of biomarkers detected according to their differential expression models through studies, thereby facilitating an improved interpretation and a hypothesis generation. biological. The simulations and a real application on the transgenic HIV-1 rats of the multi-brain region demonstrate improved sensitivity, precision and biological results of the proposed method.
New insights into the molecular mechanism of Boletus edulis ribonucleic acid fraction (BE3) concerning antiproliferative activity on human colon cancer cells.
Effects of the assessment of resveratrol on the transcription of ribonucleic acid of human telomerase of the telomerase of human telomerase in human glioblastoma.
CONTEXT
Glioblastoma (GBM) is the most common and aggressive brain tumor, which has a bad prognosis despite the advent of different therapeutic strategies. There are many molecular biomarkers to contribute to the diagnosis, prognosis and forecasting the response to current therapy in GBM. One of the most important potentially valuable markers is the immortalization-specific marker or immortalization named “HTTERT messenger ribonucleic acid” the key subunit of the telomerase enzyme, expressed in more than 85 % of cancer cells, despite the majority of normal somatic cells. In this study, we investigated the effects of Resveratrol (RSV) on this mRNA marker level, which led to the progression of cancer.
Methods
The U-87MG cell line was obtained from the Iran Pasteur Institute and treated with various concentrations of 0-160 μg / ml of RSV and at different time points (24, 48 and 72 h). To evaluate the viability of U-87MG cells, the standard 3- (4,5-dimethiazol-2-yl) -2.5-diphenylthazolium dosage was performed. The real-time polymerase chain reaction (RT-PCR) was used for a comparative and quantitative assessment of the reverse transcriptase of human tellyrase (HERT) Copy Number of mRNA against the control group-untreated.
Description: A competitive ELISA for quantitative measurement of Monkey mRNA export factor(RAE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey mRNA export factor(RAE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey mRNA export factor(RAE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Rat ovary tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat ovary tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the ovary tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The ovary tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human ovary tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human ovary tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the ovary tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The ovary tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Fetal human ovary tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human ovary tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the ovary tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The ovary tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human ovary tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human ovary tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated ovary tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated ovary tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human ovary tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human ovary tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated ovary tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated ovary tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Tissue, Array, Human Tumor, Different Types of Tumor, Multi, tissue VI (5), ovary, control, uterus, control, normal placenta (matched with mRNA blots) (Paraffin)
Tissue, Array, Human Tumor, Different Types of Tumor, Multi, tissue VI (5), ovary, control, uterus, control, normal placenta (matched with mRNA blots) (Paraffin)
Description: This cell lysate is prepared from rat ovary tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse ovary tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: Human ovary tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human ovary tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated ovary tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated ovary tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
RESULTS
The results of our survey suggest that RSV effectively inhibited cell growth and caused cell death to the dose-dependent dose (p <0.05) and not the time dependent on time (p >> 0.05), in vitro. Interestingly, an RT-PCR quantitative analysis has demonstrated that at half-concentration of inhibition, RSV significantly decreased the expression of HTERT mRNA, the catalytic subunit of the telomerase enzyme, which leads to the prevention of cell division and tumor progression.
