Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.

Dec 14, 2020 Antibodies

Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.

The cold-inducible ribonucular acid binding protein (CIRP) has been identified to play a role in the antipoptotic effect of hypothermia. We sought to investigate CIRP renoprotection in a rat model of a deep hypothermic circulatory arrest.
The overexpression and reversing of the CIRP have been obtained in vivo by directly injuring the vectors of lentivirus containing lentivirus lentivirus (PL) / of the internal fluorescent protein / green fluorescent protein (GFP) -CIRP or hairdressing RNA PL / short) / FF-FREE Inductible RNA binding protein (F-CIRP) -a in the renal parenchyma of 7-day rats before the deep hypothermic circulatory arrest under ultrasound guidance.

The vehicles or vectors of control lentiviruses were given to the control group or the control vector group, respectively. The renal function and activity of apoptosis have been evaluated by serum Cystatin C, the lipocalin of serum gelatinasse / gelatinasse and the deoxynucleotidine terminal of gelatinneity of the gelatinque 2′-deoxyudidine, the dosage of the Nick-end labeling of 5′-triphosphate at 24 hours after surgery. The expression of CIRP messenger RNA (mRNA) was evaluated by a quantitative real-time polymerase chain reaction. The expression of CIRP proteins and Caspase 3 has been tested by Western blot.

Compared to the SHAM Group, the Rats of the control group showed an increased expression of CIRP mRNA, CIRP protein, Caspase 3 and apoptotic frequency (p <0.01). However, in relation to the control group, the Rats of the PL / IRES / GFP-CIRP Group showed a significant decrease in CASPASE 3 activities and apoptosis, while increasing the increased expression of mRNA and protein. CIRP. Rats in the PL / SHRNA / F-CIRP-A group showed increased activities of CASPASE 3 and apoptosis and a decrease in a further decrease in CRNA and CIRP protein (p <0, 01), compared to the control group. The renal function was particularly protected in the PL / IRES / GFP-CIRP group and altered in the PL / SHRNA / F-CIRP-A group.
Our results suggest that the CIRP exerts a robust renoprotective effect by inhibiting apoptosis in the rat model of a deep hypothermic circulatory arrest.

Storage of long-term room temperature of dry ribonucleic acid to be used in the ARN-SEQ analysis.

 

RNA is an essential biological material for genomics and translational medicine research. As such, its storage of Biobanking is an important field of study. Traditionally, long-term storage in the cold (usually freezers or liquid nitrogen) is used to maintain high quality RNA (in terms of quantity and integrity). The conservation of the ambient temperature (RT) provides a cold alternative, which is in the grip of serious problems (mainly costs and security), for the long-term storage of RNA. In this study, we evaluated the performance of several RT storage procedures, including the Imagene’s RNASHELL®, where RNA is dried and maintained protected from the atmosphere and vacuum drying of RNA with such additives. that the Imagene stabilization solution and a house -made of trehalose solution.

This evaluation was completed by accelerated (equivalent to 10 years for Rnasell) and real-time studies (4 years). To verify the quality and integrity of RNA, we used RNA and RNA Integrity Number Values. Our study shows that the isolation of the atmosphere offers a higher protective effect for the storage of RNA with respect to vacuum drying and demonstrates that Rnasell allows a satisfactory RNA quality for long-term RT storage. Thus, the quality of the RNA could meet the demand for downstream applications such as RNA-SEQ.

 Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.
Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.

HASHINGDOWN OF IL-6 ribonucleic acid interference improves the effectiveness of cisplatin in laryngeal cancer stem cells by regulating the regulation path of the IL-6 / STAT3 / HIF1 regulatory channel.

CONTEXT
Cisplatin has been used in the treatment of many cancers, including larynx cancer; However, its effectiveness can be reduced due to the development of drug resistance. This study was intended to determine whether the removal of interleukin-6 (IL-6) can enhance the efficacy of cisplatin in larynx cancer stem cells (CSC) and the potential involvement of the signal transducer and the activator of transcription 3 (STAT3) and the hypoxia 1 inducible factor (HIF1) for this purpose.

Methods
The sorting cells of the fluorescence activated cells were identified in the sorting cells activated by fluorescence. The mRNA and IL-6, STAT3 and HIF1 phrases were examined with respectively the reaction of the polymerase chain in real time and the western ball. Cell proliferation was measured by MTT dosage. Turmoricity was measured by a colony formation dosage and an invasion was determined by a cell invasion test. Apoptotic cells have been counted by flow cytometry. Immunohistochemistry was performed to detect immunoreactive IL-6, STAT3 and HIF1 cells in xenograft.

RESULTS
IL-6, STAT3 and HIF1 mRNA and protein levels have been significantly increased in HEP2-CSC with respect to those of HEP2 cells. The application of SIRNA-IL-6 to Hashdown IL-6 resulted in a significant decrease in IL-6, STAT3 and HIF1 mRNA and protein rates. IL-6 HASHINGDOWN reduced cell proliferation, tumorigenetness and invasion and increased apoptosis within CSC. Improved deletion degrees of these parameters were observed when the IL-6 return was associated with cisplatin in these CSCs. The results of the Xenograft study showed that the combination of reversal and cisplatin IL-6 inhibited the growth of xenografts with respect to that obtained in the single injected group of cisplatin.

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EUR 351
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EUR 351
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EUR 351
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EUR 351
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Total RNA from Human Tumor Tissue: Stomach

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EUR 351
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EUR 351
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EUR 351
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EUR 351
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R1235268-10 10 ug
EUR 351
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R1235274-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Corpus Callosum

R1236045Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Frontal Lobe

R1236051Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Occipital Lobe

R1236062Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Parietal Lobe

R1236066Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Postcentral Gyrus

R1236072Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Precentral Gyrus

R1236073Alz-10 10 ug
EUR 351
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Total RNA from Alzheimer's Disease: Temporal Lobe

R1236078Alz-10 10 ug
EUR 351
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Total RNA from Congenital heart disease: Heart

R1236122Hd-3 50 ug
EUR 351
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EUR 351
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EUR 351
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R1236138Hd-2 50 ug
EUR 351
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Total RNA from hypertension: Heart Ventricle, right

R1236139Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Adipose

R1334003-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Bladder

R1334010-50 50 ug
EUR 303
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Brain

R1334035-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Colon

R1334090-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Heart

R1334122-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Liver

R1334149-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Lung

R1334152-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Pancreas

R1334188-50 50 ug
EUR 341
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Placenta

R1334200-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Rectum

R1334206-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Spleen

R1334246-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Stomach

R1334248-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Testis

R1334260-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Adipose

R1434003-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Bladder

R1434010-50 50 ug
EUR 303
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Brain

R1434035-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Colon

R1434090-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Heart

R1434122-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Liver

R1434149-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Lung

R1434152-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Ovary

R1434183-50 50 ug
EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Pancreas

R1434188-50 50 ug
EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Placenta

R1434200-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Rectum

R1434206-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Immunoreactive IL-6, STAT3 and HIF1 Cell numbers were significantly reduced in the tumor tissues of IL-6. IL-6, STAT3 and HIF1 Immunoreactive cell accounts have been further reduced in the tissues where the overturning IL-6 has been combined with cisplatin treatment with respect to fabric cisplatin only.

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